Pig CRISPR Knockout Pooled Library

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Pig CRISPR Knockout Pooled Library

Pig CRISPR Knockout Pooled Library

Catalog# LIBR-PG001-P002 LIBR-PG001-P100 LIBR-PG001-P200 LIBR-PG001-P500

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Instruction

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The CRISPR knockout library targets 22,661 genes across the pig genome and contains a total of 123,951 knockout plasmid vectors, of which 5 different gRNA vectors are designed for each gene, in addition to 2,000 control vectors targeting intergenic sequences. The library uses LentiCRISPR v2 as the backbone, which is a single-plasmid system that expresses both gRNA and Cas9 gene and can be used directly.
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Product Information

Product Name
Pig CRISPR Knockout Pooled Library
Organism
Pig
Library Type
Knockout Library
Plasmid System
Single-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
20661
gRNA Number
123951
Non-targeting gRNA Number
2000
Label
Puro
Vector Formula
LentiCRISPR v2 vector formula
Click to view the full image
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
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Cas9 Stable Cell Line
The Cas9 cell lines in our cell bank can stably express Cas9 protein. So gene knockout can be achieved by transfecting gRNA. Simultaneously transfecting gRNA and donor DNA can achieve gene knock-in/point mutation, effectively improving experimental efficiency.
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